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First things first, verify that your input file format is supported by IGV and that you have the correct index files. Make sure you are using the correct file type. IGV supports various file types, including BAM, CRAM, VCF, BED, and more. For BAM and CRAM files, you need a corresponding BAI or CRAI index file, respectively. For VCF files, you'll need a TBI index file. The index file must be in the same directory as the data file, and must be named correctly. For instance, a BAM file named "example.bam" should have a BAI index named "example.bam.bai". If the index file is missing, you'll need to create it. Also, check that the file extensions are correct, and that there are no typos in the file names. IGV requires the index file to be correctly named and located in the same directory as the data file. If the index file is missing or corrupted, you'll need to either regenerate it or ensure the correct index file is present. Sometimes, the index file might have become corrupted, so you may need to recreate it. Check that your files are not corrupted, and that you can access the files.
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*Fermented ingredients* are used frequently. *Tempeh* (fermented soybean cakes) and *tauco* (fermented soybean paste) are examples of ingredients that contribute umami and depth of flavor. These ingredients add a unique character, providing flavors that are not found elsewhere. Fermentation is a time-honored tradition in Indonesian cooking, dating back centuries.
